Detection of Bartonella (Rochalimaea) quintana by routine acridine orange staining of broth blood cultures
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چکیده
منابع مشابه
Acridine orange staining and radiometric detection of microorganisms in blood cultures.
To determine whether acridine orange (AO) staining of blood cultures could be used as a substitute for blind subculture when used in conjunction with the BACTEC system (Johnston Laboratories, Inc., Towson, Md.), the two methods were compared on all BACTEC-negative specimens. Since blind subcultures were routinely performed in our laboratory on days 2 and 6 of incubation, AO staining was also pe...
متن کاملComparative study of subculture, Gram staining and acridine orange staining for early detection of positive blood cultures.
In view of the importance of a rapid aetiological diagnosis in septicaemia, we compared the results of subculture, Gram staining and acridine orange staining in the detection of positive blood cultures. The study was based on 1013 blood cultures of which 138 were positive by culture. The three techniques were applied 12 h after the specimen was taken in 210 instances, at 24 h in 540 instances a...
متن کاملStaining with an Acridine Orange Derivative for the Detection of Mycoplasmas in Cell Cultures
• Fischer 0., Dagmar Zendulkova: Staining With an Acridine Orange Derivative for the Detection of Mycoplasmas in Cell Cultures. Acta vet. Brno, 62,1993: 49-53. 24 cell cultures were examined for the presence of mycoplasmas by fluorescence methods using 3-amino-6-methoxy-9-(2-hydroxyethylamino) acridine (AMHA) or bisbenzimide 33258 (Hoechst), and by culture in liquid media containing glucose or ...
متن کاملDetection of Campylobacter jejuni invasion of HEp-2 cells by acridine orange-crystal violet staining.
متن کامل
Murine antibody responses distinguish Rochalimaea henselae from Rochalimaea quintana.
Rochalimaea henselae causes persistent bacteremia, bacillary angiomatosis, and parenchymal bacillary peliosis. Detection of a specific antibody response to R. henselae infection may represent an alternative to cultivation as a means of diagnosis. We assessed the specificity of induced murine antibodies for antigens from R. henselae and the closely related species R. quintana. Groups of CD-1 mic...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1994
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.32.6.1492-1496.1994